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2018 Detection of the European pond turtle (Emys orbicularis) by environmental DNA: is eDNA adequate for reptiles? — Raemy M & Ursenbacher S — Amphibia-Reptilia 39 doi> 10.1163/15685381-17000025 Recent studies have demonstrated the potential of combining molecular technologies with environmental sampling to detect various vertebrate species in aquatic ecosystems. The European pond turtle (Emys orbicularis) is a threatened and elusive aquatic reptile with shy behaviour. We aimed to develop and evaluate a methodology to detect the presence of this secretive aquatic reptile in ponds from environmental water samples. First, we determined that reptilian DNA can be isolated and amplified from water samples in artificial and natural ponds with known turtle density. Then we compared the potential of two water sampling methods (through filtration or precipitation) and found no significant differences between these approaches. Finally, we demonstrated that the eDNA concentration detected is not correlated with the number of E. orbicularis individuals or biomass. Detection of eDNA was higher in artificial ponds with small volumes of water or in the shallow waters of natural ponds. The eDNA-based methodology aims to detect the presence of specific species, even at low density, with better accuracy than visual observation. However, our study indicates that this method of population monitoring should be applied with caution to aquatic reptiles. Keywords: aquatic ecosystems - environmental DANN - reptiles - species detectability |
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2017 Hybridisation between turtle subspecies: a case study with the European Pond Turtle (Emys orbicularis) — Raemy M, Fritz U, Cheylan M & Ursenbacher S — Conservation Genetics 18: 287–296 doi> 10.1007/s10592-016-0901-y Genetic introgression has recently become an important concern for conservation genetics as it can occur between rare and related common species, between various genetic groups and between individuals from different origins. Our aims were: i) to determine whether hybridisation or introgression occurs between Emys orbicularis subspecies in a natural contact zone in France; ii) to determine the geographic extent of the contact zone between distinct subspecies in France; iii) to evaluate at which level introgression occurs, and finally; iv) to evaluate whether combining mitochondrial and nuclear genetic markers reflects the same contact zone as when only one genetic marker is used. Introgression was evaluated by genotyping biparentally inherited microsatellites and sequencing the maternally inherited cytochrome b gene of French populations. We demonstrated strong introgression between subspecies under natural conditions in the old contact zone in southern and eastern France. Our results corroborated that introgression reflects past natural events, but also demonstrated that human impact has altered these patterns. We finally confirmed that the combination of mitochondrial and nuclear genetic markers is more appropriate to reveal introgression than the use of only one genetic marker Keywords: conservation genetics - France - hybridization - introgression |
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2013 European pond turtle (Emys orbicularis) conservation projects in Switzerland — Raemy M, Monney J-C & Ursenbacher S — Herpetological Notes 6: 111–112 Keywords: Emys orbicularis - Switzerland - conservation |